Bio-Based Potential of Phenolic-Rich Fabaceae Seeds (<i>Gonocytisus angulatus, Podocytisus caramanicus</i>): Chemical Standardization, Functional Screening, and Molecular Insights

BAYRAK, Burak; GÜNEŞ, Ferhat; AKYÜZ, Beyzanur; Beyza Ozer, Elif; Abdulqader, Amenah; Civas, Ayse; Kucuksu, Isranur; Sonmez, Hatice; Cecen, Omer; AYDIN, BİLGE; Coban, Furkan; Yuca, Hafize; Karakaya, Songul

doi:10.1002/cbdv.71739

Bio-Based Potential of Phenolic-Rich Fabaceae Seeds (<i>Gonocytisus angulatus, Podocytisus caramanicus</i>): Chemical Standardization, Functional Screening, and Molecular Insights

BAYRAK B., GÜNEŞ F., AKYÜZ B., Beyza Ozer E. B., Abdulqader A. I., Civas A., ...Daha Fazla

CHEMISTRY & BIODIVERSITY, cilt.23, sa.9, 2026 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 23 Sayı: 9
Basım Tarihi: 2026
Doi Numarası: 10.1002/cbdv.71739
Dergi Adı: CHEMISTRY & BIODIVERSITY
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, EMBASE, MEDLINE, Natural Science Collection (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest), Materials Science & Engineering Collection (ProQuest), Technology Collection (ProQuest)
Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

This study comparatively evaluated the phytochemical composition and bioactivities of methanolic extracts and aqueous decoctions obtained from seeds of two Fabaceae taxa (Gonocytisus angulatus, Podocytisus caramanicus). Aqueous decoction yielded higher extraction efficiencies (up to 39.20%), whereas methanolic extraction recovered a broader range of phenolic compounds. Phytochemical analysis revealed that flavonoids and phenolic acids were the dominant constituents, with chlorogenic acid, rutin, quercetin, kaempferol, and gallic acid as the major compounds, particularly enriched in methanolic extracts. In vitro assays demonstrated moderate enzyme inhibitory effects. The highest alpha-glucosidase inhibition was observed in the methanolic extract (20.22%), while alpha-amylase inhibition reached 30.11%. Anticholinesterase activity was also weak, with maximum acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition values of 15.27% and 19.93%, respectively. In contrast, antioxidant activity was more pronounced, with ABTS radical scavenging reaching 97.40%, comparable to the reference standard. Molecular docking supported these findings by indicating favorable binding interactions of major phenolic compounds with target enzymes.