The prevalence of bla(KPC), bla(NDM), bla(OXA-48), blame and bla(VIM) genes and phenotypic carbapenemase production was investigated in ESBL-producing Enterobacteriaceae strains isolated from 150 raw cow milk samples collected from the Black Sea region of Turkey. A total of 47 phenotypic ESBL producers, 41 Escherichia coli and six Klebsiella pneumoniae, were investigated in terms of carbapenemase activity. The presence of carbapenemase genes was investigated using five single PCR assays. Seven of the 47 isolates were found resistant to imipenem and/or meropenem. PCR assays revealed that 33 of the 47 isolates were found to harbour at least one of the analysed carbapenemase genes. For bla(KPC), bla(OXA-48), bla(IMP) and bla(VIM )genes, BLAST search indicated 97.9%, 97.2%, 97.1% and 97% nucleotide identity to corresponding sequences, respectively. Carbapenemase-producing E. col/ and K. pneumoniae were found higher in milk compared with studies conducted worldwide and carbapenemase production was associated with bla(OXA-48) and bla(KPC). (C) 2022 Elsevier Ltd. All rights reserved.