Introduction - Ticks and tick-borne diseases are a global problem. Cypermethrin and flumethrin are synthetic pyrethroid acaricides widely used for tick control in cattle. As is the case for all families of acaricides, pyrethroid resistance in ticks is one of the most important problems in cattle husbandry by causing major health problems and economic losses. Therefore, continuing studies of the susceptibility to resistance of currently used acaricides is essential for effective tick control programs. In addition, measuring blood and milk concentrations of applied acaricides is essential for the evaluation of their efficacy against ticks and potential toxicity to animals and humans.