It is aimed to detect allergen determination via serum specific immunoglobulin E (IgE) for the first time in dogsdiagnosed as Atopic Dermatitis (AD) from Samsun and vicinity within this present study. Twenty one dogs withdifferent age, breed and gender diagnosed with AD based up on the Favrot’s criteria. Serum samples of the dogswere analyzed by detection of serum specific IgE for determination of 20 different allergens. The effects ofgender, age and the feeding routes for those 20 different allergens separately were analyzed. Based up on thegrading of the allergens detected in 21 dogs with AD from Samsun and vicinity; results reflects thatDermatophagoides farinae was stated as first degree within a very high seropositivity rate (95%). Moreover, in varyingdegree of seropositivitiy rates of other allergens were also detected. Females showed statistically (p<0.05) higherpositivity rate to the 20th allergen, Ctenocephalides spp. more than males. Furthermore, we did not found anysignificant effects of age and feeding routes on the allergens in the dogs with AD (p>0.05). Dogs with AD fromSamsun and vicinity had very high serum specific IgE levels against to the house dust and storage mites.