In this study, 14 fungal samples were isolated from tobacco (Nicotiana tabacum L.) cultivated in Samsun-Turkey, corresponded with binucleate Rhizoctonia (BNR) anamorphic criteria. Of these, 10 isolates were identified as BNR AG-A, one as BNR AG-Fa and 3 as BNR AG-Fb at the results of anastomosis tests and rDNA-ITS phylogenies. Five of 10 AG-A isolates were found as inducers for root rot of tobacco, whereas 5 AG-A isolates and AG-Fa, Fb isolates were found as slightly virulent or non-pathogenic. This study presents the first report for B.N. Rhizoctonia AG-A as a pathogen for tobacco.