Adsorption of lysozyme from aqueous solutions by a novel bentonite-tyrptophane (Bent-Trp) microcomposite affinity sorbent

Kalburcu T., Tabak A., Ozturk N., Demir M. N., Akgol S., Çağlar B., ...More

JOURNAL OF MOLECULAR STRUCTURE, pp.156-162, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2015
  • Doi Number: 10.1016/j.molstruc.2014.11.055
  • Journal Name: JOURNAL OF MOLECULAR STRUCTURE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.156-162
  • Keywords: Affinity chromatography, Bentonite-tyrptophane microcomposite, Lysozyme, TG-DTG/DTA, XRD
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

This study deals with developing bentonite-tryptophan (Bent-Trp) microcomposite affinity sorbent (38-105 gm) for lysozyme adsorption from aqueous solutions. The structural analysis results of Bent-Trp microcomposites obtained from X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), thermal (TG-DTG/DTA) and elemental analysis techniques clearly pointed out that the pseudo-biospecific affinity ligand L-Tryptophan was penetrated into interlayer space of the bentonite by diplacing water molecules and an elemental analysis of immobilized L-Tryptophan for nitrogen was 541.3 mu mol g(-1) bentonite. The effects of initial concentration, pH, and temperature on the adsorption efficiency of this sorbent were also studied in a batch system. The maximum amount of lysozyme adsorption from aqueous solution was determined as 365.16 mg/g at pH 10.0 and 1.25 mg/mL initial concentration of lysozyme, while the non-specific adsorption of lysozyme was very low (4.21 mg g(-1) sorbent). We concluded that Bent-Trp microcomposite affinity sorbents could be repeatedly applied for lysozyme adsorption without significant losses in the adsorption capacity. (C) 2014 The Authors. Published by Elsevier B.V.