Research Information System
Molecular Biology Research Communications, vol.12, no.3, pp.109-115, 2023 (Peer-Reviewed Journal)
In this study, the relationship between RORA 23bp indel genotype and allele frequency with
twin pregnancy, fertility, live weight and milk yield in 106 female Akkaraman ewes raised in
Elazığ province was investigated. In the study conducted in Elâzığ province, 10ml milk was
collected from 106 Akkaraman sheep and DNA was extracted from these milk. In RORA 23bp
indel genotype frequency, DD genotype was found more than ID and II genotypes and RORA
23bp indel ın allele frequency, the D allele was found to be higher than the I allele. In both the
first and second parity, the twinning rate was found to be lower. In both the first and second
parity, the twinning rate was higher in the DD genotype, and it was observed that this genotype
promınated mıddle lıvestock weıght and mılk yıeld. According to the results of our study,
mutations in the RORA gene, which is a gene affecting reproductive efficiency in sheep, do not
have a positive effect on fertility and twinning rate in Akkaraman sheep. To sum, this study
provided theoretical references for the comprehensively research of the function of RORA gene
and the breeding of Akkaraman Sheep. The 23-bp indel variants can be considered as molecular
markers for litter size of sheep for marker-assisted selection breeding.