Isolation, characterization and antiproliferative activity of secondary metabolites from tanacetum alyssifolium (Bornm.) grierson

Creative Commons License

KÖKSAL E., Ulutaş Y., ŞİMŞEK S., Bayraktar T., ALTAY A., ÇATIR M., ...More

Records of Natural Products, vol.15, no.2, pp.122-129, 2021 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 15 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.25135/rnp.
  • Journal Name: Records of Natural Products
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, ABI/INFORM, CAB Abstracts, EMBASE, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.122-129
  • Keywords: Cytotoxicity, MCF-7 cell line, Sesquiterpene lactones, Tanacetum alyssifolium
  • Ondokuz Mayıs University Affiliated: No


Secondary metabolites of Tanacetum alyssifolium (Bornm.) Grierson (Asteraceae) were investigated for the first time. Thirteen compounds including axillarin (1), Luteolin-7-O-β-glucoside (8) and rutin (13) as flavonoids, fraxetin (7), isofraxidin (9), isofraxidin-7-O-glucoside (11) and fraxidin (12) as coumarins, tatridin A (2), altissin (3), tamirin (4) and tanachin (5) as sesquiterpene lactones and 2,4-dihydroxy-6-methoxy acetophenone (6) and picein (10) as acetophenone derivatives were isolated from the methanol extract of the species. The structures of all isolated compounds were elucidated by 1D and 2D-NMR techniques and by comparing with the literature data. In addition, in vitro cytotoxic activity of all isolated compounds was evaluated against breast cancer MCF-7 cell line by XTT assay. Sesquiterpene lactones; tatridin A (2), tamirin (4) and tanachin (5) were found to be the most cytotoxic molecules against MCF-7 cell line.