Flow-injection spectrophotometric determination of copper (II) ion at low μg L-1 levels

Asan A.

International Journal of Environmental Analytical Chemistry, vol.79, no.2, pp.111-119, 2001 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 79 Issue: 2
  • Publication Date: 2001
  • Doi Number: 10.1080/03067310108035903
  • Journal Name: International Journal of Environmental Analytical Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.111-119
  • Keywords: Copper(II) determination, Flow-injection, N,N′-o-phenylenebis(salicylaldimine), Spectrophotometry
  • Ondokuz Mayıs University Affiliated: Yes


A highly selective, sensitive and fast determination of trace amounts of copper (II) ion is described. The method is based on the formation of the coloured Cu(II)-N,N′-o-phenylenebis(salicylaldimine) (PBS) complex, which is then selectively monitored at 415 nm. To increase the interaction between copper(II) and the colour forming reagent and preconcentrate copper(II), a microcolumn containing strong cation-exchange resins was placed between the injection manifold and the spectrophotometer. The linear range of the proposed method was 25-100 μg L-1, and the limit of detection (signal-to-noise ratio = 2) was < 1 μg L-1 for 20 μL injection volume of copper (II) ion solution at a sampling rate of > 50 h-1. The relative standard deviation at the level of 25-100 μg L-1was 0.24 % for n = 5. The precision and accuracy of the method was checked by analysis of a certified reference material, and the applied to fiver and sea water samples.