Effect of Enzyme Supplementation and Fermentation Time on the Nutritional Composition of Olive Pomace Fermented by Lactobacillus acidophilus


Güngör E., Özlü Ş., Güngör B., Altop A., Erener G.

TURKISH JOURNAL OF AGRICULTURE: FOOD SCIENCE AND TECHNOLOGY, cilt.13, sa.09, ss.2759-2763, 2025 (Hakemli Dergi)

Özet

Nutritional changes of olive pomace during solid-state fermentation by Lactobacillus acidophilus with different fermentation times and enzyme supplementation were investigated in this study. A 3 × 2 factorial design was performed to investigate the effect of fermentation time (3, 5, and 7 days) and enzyme supplementation (with or without) on the nutritional composition of olive pomace, with five replicates in each group. Before fermentation, olive pomace was ground to a 1 mm particle size and sterilized after mixing with distilled water at 121°C for 15 min. The enzyme mixture (phytase, xylanase, and beta-glucanase) sterilized by UV lights was added to half of the fermentation medium. The olive pomace was inoculated with Lactobacillus acidophilus and incubated at 30°C for 3, 5, and 7 days. Solid-state fermentation using Lactobacillus acidophilus increased the crude protein and crude fiber content, decreased the ether extract content, and had no influence (P=0.094) on the ash content of olive pomace. The highest crude protein content was observed in fermented olive pomace for 3 days with enzyme and for 5 days without enzyme supplementation (P<0.001). Fermented olive pomace for 7 days without enzymes had the lowest crude fiber content (P<0.001). The highest ether extract level was found in fermented olive pomace for 3 days with or without enzyme. Enzyme supplementation caused higher ash level in fermented olive pomace. Besides, a linear increase was observed in ash content with increased fermentation time. The highest ash level in fermented olive pomace was found at 7 days of fermentation. The result showed that Lactobacillus acidophilus improved the nutritional composition of olive pomace in solid-state fermentation. Considering the highest crude protein content and relatively lower crude fiber and higher ether extract content, 3 days of fermentation with enzyme is the most effective fermentation conditions with Lactobacillus acidophilus for olive pomace