Akademik Veri Yönetim Sistemi
V. International Enzyme and Bioprocess Days, İzmir, Türkiye, 27 Ağustos 2024, ss.65, (Özet Bildiri)
Esterases are enzymes with broad substrate specificity that catalyze the formation and breakage of ester
bonds in molecules. They are employed in biotechnological applications, particularly in degradation of
xenobiotics, ripening of dairy products, removal of oily contaminants from fabrics. However, one of
the factors limiting their widespread use is their production cost. Therefore, the presented study aimed
to decrease the production cost by statistically optimizing the process under conditions using sunflower
seed meal as an inducer. Medium components for esterase production were screened using Plackett–
Burman design in a cold-adapted bacterium, isolated from Kackar Mountains/Türkiye and designated
as Janthinobacterium sp. L21D134 according to 16S rRNA gene sequence analysis. Model p-, R2,
Adjusted-R2, and Prediction-R2 values were found to be 0.0390, 0.9997, 0.9972, and 0.9639,
respectively. Result showed that glucose, peptone, yeast extract, magnesium sulphate, K2HPO4, Triton
X-100, malt extract, and ammonium sulphate had positive effects on enzyme production. Among them,
effect of pepton, yeast extract and glucose were significant according to t-values of effects. Then, four
medium components (3 significant and 1 inducer) were optimized using Response Surface
Methodology-Central Composite Design . Models p-, R2, Adjusted-R2, and Prediction-R2 values were
< 0.0001, 0.9875, 0.9759, and 0.9455, respectively. According to the numerical optimization, the
optimal concentrations of glucose, peptone, yeast extract, and SSM are found to be 4.15 g/L, 5.44 g/L,
8.98 g/L, and 21.33 g/L, respectively. Maximum esterase titer is predicted to be 13.64 U/mL with a
desirability value of 1.0 in the optimized fermentation medium.