Objective: This study was planned to determine the level of knowledge about breast cancer (BC) and attitudes and practices towards BC infemale healthcare providers. Thereby, it was aimed to identify the level of awareness, which is intended to be improved in the community,among female healthcare providers; the topics of failure; attitudes and practices towards screening programs; and factors involved in theseareas. Method: The study sample consisted of 266 female healthcare providers. In all participants, a 29-item questionnaire was completed bythe face-to-face interview method. The questionnaire included items about sociodemographic characteristics, and level of knowledge,attitudes and practices in BC. Each correct answer was rated as one point while no score was assigned to incorrect answers which wereexcluded. Data were analyzed by SPSS version 22.0 (SPSS Inc., Chicago, IL, USA). Results: The mean age was 30.29 ± 0.55 years. Ofsubjects, 47.7% (n=127) were physicians, 48.1% (n=128) were nurses while 4.2% (n=11) were other healthcare providers. Significantdifferences were found in the level of knowledge according to marital status, educational level and age. Of the factors affecting participationin screening programs, marital status, educational level, age, and duration of a professional career. It was found that the level of knowledgeregarding BC was high while the participation rate for screening programs was low among subjects.Conclusion: It was found that knowledgelevel was high while the participation rate for screening programs were rather low among female healthcare providers. This shows that thereshould be studies in order to increase the participation of female healthcare providers in screening programs.