Comparison of the antimicrobial activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine
Journal of Conservative Dentistry, vol.19, no.6, pp.569-572, 2016 (Scopus)
- Publication Type: Article / Article
- Volume: 19 Issue: 6
- Publication Date: 2016
- Doi Number: 10.4103/0972-0707.194018
- Journal Name: Journal of Conservative Dentistry
- Journal Indexes: Scopus
- Page Numbers: pp.569-572
- Keywords: Agar diffusion test, Biodentine, direct pulp capping, endodontics, mineral trioxide aggregate-Angelus
- Ondokuz Mayıs University Affiliated: Yes
Abstract
Purpose: To compare the antimicrobial activity of the tricalcium silicate-based Biodentine (BD) and mineral trioxide aggregate (MTA)-Angelus cement with the aid of agar diffusion test. Materials and Methods: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecium were inoculated in the Brucella liquid medium and were incubated at 37°C for 24 h. Thereafter, 100 >μl of the liquid culture of bacteria inoculated in the Mueller-Hinton agar with spread plate technique. Petri plates were dried in room temperature. For every microorganism, 3 petri plates were prepared (12 in total). In the medium, in every petri plate, 2 holes with 5 mm diameter and 2 mm depth were made. Afterward, BD and MTA-Angelus were filled into these holes under aseptic conditions according to the instructions of the manufacturing company. Then, the plates were kept in the incubator at 37°C for 24 h, and the diameters of the inhibition zones were measured with a digital caliper. Results: Inhibition zones formed by BD against E. coli and S. aureus were significantly larger than the zones formed by MTA-Angelus (P < 0.05). However, the inhibition zones formed by MTA-Angelus against P. aureus and E. faecium were larger than the zones formed by BD (P < 0.05). Conclusion: Within the limits of the present study, tricalcium silicate-based MTA-Angelus and BD have antimicrobial activity against E. coli, S. aureus, P. aureus, and E. faecium.