Assessment of the Enzyme Inhibitory Activities of Salvia dorystaechas and Its Phytochemical Analysis by RP-HPLC

Özüpek B., Pekacar S., Saltan N., Yıldız G., Gülcan Z., Deliorman Orhan D., ...Daha Fazla

PHARMACEUTICAL CHEMISTRY JOURNAL, cilt.58, sa.11, ss.1734-1741, 2025 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58 Sayı: 11
  • Basım Tarihi: 2025
  • Dergi Adı: PHARMACEUTICAL CHEMISTRY JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core
  • Sayfa Sayıları: ss.1734-1741
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Salvia dorystaechas is an endemic species found around Antalya in Anatolia, and in this study, the antidiabetic, antiobesity, antihyperlipidemic, and antimelanogenic potentials of the extracts (methanol, ethyl acetate, and chloroform) obtained from the root and aerial parts of the plant were evaluated using in vitro enzyme models (α-glucosidase, α-amylase, pancreatic lipase, cholesterol esterase, and tyrosinase). Root ethylacetate (IC50 6.53 ± 0.19 μg/ml) and aerial part chloroform extracts (IC50 24.14 ± 1.76 μg/ml) inhibited the pancreatic cholesterol esterase enzyme, as much as, or even more potently than simvastatin (IC50 20.48 ± 2.70 μg/ml). All extracts showed weak or no inhibitory activity against other enzymes. RP-HPLC analysis results showed that S. dorystaechas root ethyl acetate extract, which has the strongest pancreatic cholesterol esterase inhibitory activity, can be standardized with rosmarinic acid (3.164 ± 0.001 mg/g plant) and caffeic acid (1.118 ± 0.000 mg/g plant). As a result, it can be predicted that this plant, which is also commonly consumed as a tea, can be evaluated as a medicinal tea or medicinal herbal product with further studies due to its strong cholesterol-lowering effect.