Akademik Veri Yönetim Sistemi
Türk Doğa ve Fen Dergisi, cilt.14, sa.3, ss.183-191, 2025 (Hakemli Dergi)
Berberis thunbergii, also known as Japanese dogwood, has many medicinal properties due to its rich phytochemical components. Therefore, B. thunbergii hydroethanolic extract was investigated for its phytochemical content, antioxidant, anti-inflammatory, and enzyme inhibition activities. In LC-ESI-MS/MS and GC-MS/MS analysis, the main components of the extract were determined as chlorogenic and oleic acid, respectively. Total phenol and flavonoid amounts were found as 259.40±0.96 mg GAE/g extract and 2.60±0.57 mg QE/g extract, respectively. It was recorded that it could reduce iron as 6.38 mg TE/g extract in the FRAP test and had an activity effect of 85.26% in the DPPH֗ scavenging test. The antiinflammatory property of the extract was found to be 60.80±2.22 µg/mL, while DFS was found to be 45.23±0.68 µg/mL. Additionally, in enzyme inhibition, it was observed that it inhibited urease at a low level of 44.19±0.22 µg/mL and xanthine oxidase (XO) at a high level of 0.91±0.21 µg/mL. It was recorded that it protected DNA with a 57.55% Form I value. Based on these results, it was determined that its high phenolic content also contributes to its high radical scavenging ability. Furthermore, the high XO inhibition of this extract suggests that further detailed studies will be required to demonstrate this property. Further in vivo and in silico studies may be conducted for this purpose. Furthermore, B. thunbergii is thought to have potential applications in the food, cosmetics, and medical fields.