Principles and Practices of CRISPR-Cas9 Genome Editing Techniques
CRISPR-Cas9 as a Revolutionary Gene Editing Tool: Genome Engineering, Advances and Applications in Agriculture, CRC Press, ss.1-18, 2026
- Yayın Türü: Kitapta Bölüm / Araştırma Kitabı
- Basım Tarihi: 2026
- Doi Numarası: 10.1201/9781003518204-1
- Yayınevi: CRC Press
- Sayfa Sayıları: ss.1-18
- Ondokuz Mayıs Üniversitesi Adresli: Evet
Özet
CRISPR-Cas9 has transformed plant biotechnology by enabling precise genome editing to tackle agricultural and environmental challenges. This technology facilitates targeted genetic modifications, supported by innovative delivery methods like Agrobacterium-mediated transformation and nanoparticles. Noteworthy applications include OsSWEET14-edited rice for bacterial blight resistance and eIF4E-modified cassava to combat viral losses in sub-Saharan Africa. Additionally, synthetic biology initiatives like incorporating nif genes into wheat for nitrogen fixation and engineering C4 rice for carbon sequestration aim to reduce chemical fertilizer reliance and address climate change. However, CRISPR-Cas9 faces technical and ethical challenges, such as delivery barriers, off-target mutations, and biosafety concerns. Regulatory discrepancies between regions complicate global harmonization, and ethical issues surrounding corporate seed control raise equity concerns for smallholder farmers. Despite limitations in knock-in efficiency, the future of CRISPR-Cas9 looks promising, with advancements in delivery methods and the domestication of wild species enhancing genetic diversity. Tailored solutions to local needs, like drought-resistant maize and high-yield rice, can help build resilience against climate change. Achieving this vision will require regulatory alignment, open-source innovation, and inclusive dialogues to ensure equitable benefits, positioning CRISPR-Cas9 as a catalyst for sustainable agricultural systems.