Guaiazulene is a crystalline bicyclic sesquiterpene. Today, besides being used in herbal treatment, it is also used in skin-body care products, toothpaste, hair dyes, eye solutions and also in the treatment of nipple irritation in nursing mothers. In the study, it was aimed to evaluate the effects of Guaiazulen and its solvent dimethyl sulfoxide on the level of malondialdehyde, which is a lipid peroxidation marker, and superoxide dismutase and catalase enzyme activities, which belong to antioxidant defense systems, in Drosophila melanogaster. it was determined that dimethyl sulfoxide caused lipid peroxidation by increasing the level of malondialdehyde at all hour and dose concentrations. At the same time, it was determined that dimethyl sulfoxide caused an increase in catalase enzyme activity by increasing the radical formation, but did not affect superoxide dismutase enzyme activity. In addition, it was observed that increasing in the dose of guaiazulene or the exposure time to it decreased superoxide dismutase enzyme activity, and catalase enzyme activity at all doses and hours. It was concluded that these data support the fact that guaiazulene shows antioxidant properties. Malondialdehyde amount increased upon increasing in the exposure time to guaiazulene. And it was concluded that it caused lipid peroxidation.