Akademik Veri Yönetim Sistemi
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL, cilt.29, ss.1-7, 2019 (SCI-Expanded, Scopus)
Efficacy of the five native entomopathogenic fungi (EPFs), Beauveria bassiana, Isaria fumosorosea, Lecanicillium muscarium, Metarhizium anisopliae, and Simplicillium lamellicola, against adults of the stored-grain insect pests, Sitophilus granarius and Sitophilus oryzae (Coleoptera: Curculionidae), was evaluated under laboratory conditions at two different temperatures. Conidial suspensions (1x10(8) conidia ml(-1)) of the fungi were directly applied to both the pests in Petri dishes (2ml per dish), using a Potter spray tower. All the dishes were incubated both at 20 and 25 degrees C in 16h light/8h dark and in 75 +/- 5% relative humidity (RH). Dead individuals were counted daily, following treatments, for 7days. Lethal time values (LT50 and LT90) for EPFs were calculated. The results demonstrated that the mortality rates varied according to both the temperature and the tested EPFs. The highest effect among EPFs at (1x10(8) conidia ml(-1)) concentration on S. granarius at 20 degrees C at the end of day 7 was showed by I. fumosorosea (92.69%) and M. anisopliae (90.35%), followed by the other EPFs. Similarly, M. anisopliae and I. fumosorosea were the most effective ones with 90.48 and 84.21% mortality rates, respectively, at 25 degrees C. However, while M. anisopliae (85.68%) showed the highest effect among all the EPFs applied on S. oryzae at 20 degrees C, B. bassiana with a mortality rate of 93.66% was the most effective one at 25 degrees C. LT50 values for I. fumosorosea and M. anisopliae were 2.75 and 2.88/days, respectively, and LT90 values were 4.17 and 4.47/days, respectively, at 20 degrees C for S. oryzae. However, LT50 values for M. anisopliae on S. granarius in both temperatures were the lowest. This study indicated that M. anisopliae and I. fumosorosea had a significant potential as a biological control agent against S. granarius and S. oryzae. Further studies are necessary to evaluate the efficacy of the isolate on the pests under storage conditions.