Aim: To determine whether the total sperm count has any effect on pregnancy rates in ICSI cycles. Material and Method : This retrospective cohort study was performed on single ICSI cycles of 661 couples in two separate Assisted Reproduction Technology Centers between January 2010 and December 2012. Total sperm count and pregnancy rates were compared. Results: Pregnancy rates showed difference among total sperm count groups but there was no statistically significant difference between them. Discussion: It is shown that there are no statistically significant effect of total sperm count on pregnancy rates in ICSI cycles.