A novel actinomycete, strain DS3030(T), was isolated from a deep sediment sample, collected from the southern Black sea coast, Turkey, and was examined using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain DS3030(T) was shown to belong to the genus Saccharomonospora and to be related most closely to Saccharomonospora marina XMU15(T) (99.6 % similarity). Sequence similarities with other strains of the genus Saccharomonospora were lower than 97.0 %. The organism had chemical and morphological features typical of the genus Saccharomonospora. The cell wall of the novel strain contained meso-diaminopimelic acid, arabinose and galactose as diagnostic sugars. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant menaquinone was MK-9(H-4). Major fatty acids were iso-C-16:0, iso-C-16:0 2-OH and C(16:1)cis 9. Phenotypic data clearly distinguished the new isolate from its closest relative, S. marina XMU15(T). The combined genotypic and phenotypic data and low DNA DNA relatedness with its closest related strain reveal that strain DS3030(T) represents a novel species of the genus, for which the name Saccharomonospora amisosensis sp. nov. is proposed. The type strain is DS3030(T) (=DSM 45685(T)=KCTC 29069(T)=NRRL B-24885(T)).