Usage of color measurements obtained by modified Seliwanoff test to determine hydroxymethylfurfural

Beşir Özgeçen A., Yazıcı F., ODABAŞ M. S.

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, vol.248, no.12, pp.2995-3002, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 248 Issue: 12
  • Publication Date: 2022
  • Doi Number: 10.1007/s00217-022-04106-8
  • Journal Name: EUROPEAN FOOD RESEARCH AND TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, ABI/INFORM, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Food Science & Technology Abstracts, Hospitality & Tourism Complete, Hospitality & Tourism Index, Veterinary Science Database
  • Page Numbers: pp.2995-3002
  • Keywords: HMF, Seliwanoff test, Artificial neural network, ANN, Color, ARTIFICIAL NEURAL-NETWORKS, 5-HYDROXYMETHYL-2-FURFURAL HMF, ANTIOXIDANT ACTIVITY, PREDICTION, CORN
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

In this study, colorimetric parameters of the red color emerging as a result of the novel modified Seliwanoff test were used for the quantitative determination of HMF in some syrup samples. Three different evaluation method (color space-based method, absorbance method and artificial neural network (ANN)) were used to determine HMF amounts of samples. While "da*" and absorbance value of red color were used to obtain a linear relationship with HMF concentration, L*, a*, b*, dL*, da*, db*, dE* were used as input variables in artificial neural network evaluation. Also HPLC method was carried out as a reference method. In addition to the absorbance value, 'da*' value also showed a linear relationship with the HMF concentration (da* = 0.0425 + 0.0612 x HMF concentration, ppm); correlation coefficient: 0.9980; p value of lack of fit: 0.579). When HMF amounts obtained by each method were compared with that by reference method HPLC, a strong correlation was found (color space-based method-HPLC, r: 0.996; absorbance method-HPLC, r: 0.997 and ANN-HPLC; r: 0.997). Among the methods, ANN evaluation method was more prominent in detecting low HMF concentrations (< 4 mg HMF/kg sample).