Phenolic profile and antioxidant activity of centaurea choulettiana pomel

Azzouzi D., Bioud K., Demirtas İ., Gul F., Sarri D., Benayache S., ...More

Combinatorial Chemistry and High Throughput Screening, vol.19, no.10, pp.841-846, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 10
  • Publication Date: 2016
  • Doi Number: 10.2174/1574888x11666161102092319
  • Journal Name: Combinatorial Chemistry and High Throughput Screening
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.841-846
  • Keywords: Antioxidant activity, Centaurea choulettiana, HPLC-TOF/MS, Phenolic acids, Total flavonoids, Total phenolics
  • Ondokuz Mayıs University Affiliated: No


Aim and objective: This study aimed to quantify phenolic compounds in ethyl acetate and n-butanol extract of Centaurea choulettiana Pomel (Asteraceae) leaves and flowers; compare the antioxidant activity of their extracts, identification and quantification of their phenolic acids. Materials and methods: Both organs extracts of Centaurea choulettiana Pomel were investigated and evaluated for their potential antioxidant properties using total phenolics and flavonoids content, DPPH radical scavenging and lipid peroxidation inhibition assays. HPLC-TOF/MS analyses were carried out to identify and quantify some phenolic acids. Results: The amounts of phenolic and flavonoid content were higher in ethyl acetate extract of leaves (325.81± 0.038 mgGAE and 263.73± 0.004 mgQE /g of extract) respectively. Besides, this extract exhibited the most powerful effect on the DPPH radical scavenging activity with (96.54%), on lipid peroxydation inhibition (64.17%). Ethyl acetate extract of leaves and flowers were found to contain almost the same phenolic compounds, with the leaves having the highest values. Chlorogenic acid was detected in the n-butanol extract of flowers with the highest concentration 17.78 mg/kg plant. Conclusion: The ethyl acetate extract of leaves of Centaurea choulettiana possesses strong antioxidative properties in vitro. They are confirmed by high polyphenols and flavonoids content. The HPLC-TOF/MS analysis reveals the presence of 4-hydroxybenzoic acid, gentisic acid, chlorogenic acid, caffeic acid, vanillic acid, p-Coumaric acid, ferulic acid, salicylic acid and protocatechuic acid.