A recombinant PvpA protein-based diagnostic prototype for rapid screening of chicken Mycoplasma gallisepticum infections
VETERINARY MICROBIOLOGY, vol.129, no.1-2, pp.139-149, 2008 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 129 Issue: 1-2
- Publication Date: 2008
- Doi Number: 10.1016/j.vetmic.2007.11.028
- Journal Name: VETERINARY MICROBIOLOGY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.139-149
- Keywords: Mycoplasma gallisepticum, recombinant PvpA, serodiagnostic prototype, chicken, MONOCLONAL-ANTIBODY, ANTIGEN, CYTADHESIN, EXPRESSION, ASSAY, GENE, HEMAGGLUTININS, STRAINS, EPITOPE, CLONING
- Open Archive Collection: AVESIS Open Access Collection
- Ondokuz Mayıs University Affiliated: Yes
Abstract
Mycoplasma gallisepticum is the primary agent of chronic respiratory disease causing important economic losses in the poultry industry. Serological monitoring is essential to maintain mycoplasma-free breeder flocks and often complicated by the cross-reactions between different mycoplasma species. To overcome serological cross-reactions, a large fragment of the M. gallisepticum PvpA cytadhesin, species-specific surface-exposed protein, was produced in E. coli as a recombinant protein (rPvpA336) and used as a potential diagnostic antigen. The rPvpA336 protein possesses 336 mycoptasma-specific amino acids with relative molecular weight of 44 kDa. A deletion region of 37 amino acids was identified when compared to the wild-type PvpA protein. Immunoreactivity of the rPvpA336 protein has been demonstrated by Western blot analysis with M. gallisepticum-positive and -negative chicken sera. Furthermore, an enzymatic rapid immunofiltration assay (ERIFA) prototype based on the rPvpA336 protein has been developed and its species-specific detection capability has been demonstrated by using M. gallisepticum and/or M. synoviae-positive and -negative chicken sera. In addition to its species-specificity, the ERIFA prototype presents certain advantages such as rapidity, field-applicability and cost-effectiveness. Therefore, these advantages would make the prototype a species-specific rapid diagnostic tool of choice in the field and limited laboratory conditions for screening M. gallisepticum infections. (C) 2007 Elsevier B.V. All rights reserved.