CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation

Cardi T., Murovec J., Bakhsh A., Boniecka J., Bruegmann T., Bull S. E., ...More

Trends in Plant Science, vol.28, no.10, pp.1144-1165, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Review
  • Volume: 28 Issue: 10
  • Publication Date: 2023
  • Doi Number: 10.1016/j.tplants.2023.05.012
  • Journal Name: Trends in Plant Science
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, MEDLINE, Veterinary Science Database, DIALNET
  • Page Numbers: pp.1144-1165
  • Keywords: CA18111, CRISPR applications, CRISPR platforms, gene regulation, mutant detection, plant regeneration, PlantEd
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

The discovery of the CRISPR/Cas genome-editing system has revolutionized our understanding of the plant genome. CRISPR/Cas has been used for over a decade to modify plant genomes for the study of specific genes and biosynthetic pathways as well as to speed up breeding in many plant species, including both model and non-model crops. Although the CRISPR/Cas system is very efficient for genome editing, many bottlenecks and challenges slow down further improvement and applications. In this review we discuss the challenges that can occur during tissue culture, transformation, regeneration, and mutant detection. We also review the opportunities provided by new CRISPR platforms and specific applications related to gene regulation, abiotic and biotic stress response improvement, and de novo domestication of plants.