The green methodology is one of the most rising production methods since this strategy is simpler than other alternate strategies, eco-friendly, and offers minimal time exhaustion for the production. In this study, silver nanoparticles (AgNPs) were synthesized via the green synthesis method using Sidr honey (SH) and Rhododendron honey (RH), and their characterization was determined. A certain proportion of honey for the silver ion was prepared, and the color alteration which demonstrated the development of AgNPs was observed during the production. The characterization of AgNPs has been identified using UV-vis spectroscopy, FTIR, TEM, SEM, and EDX. As a result, SH-AgNPs and RH-AgNPs were determined with the maximum surface plasmon resonance (SPR) absorption at 443 nm and 456 nm; the average particle size of SH-AgNPs, and RH-AgNPs was 14.3 nm and 14.7 nm, respectively. SH-AgNPs (1:1-5mM) and RH-AgNPs (1:1-5mM) showed more improved antibacterial activity than others against S. aureus with an inhibition zone 14 mm. Further, the synthesized nanoparticles can be used to manufacture antibacterial drugs and may open the door to enhance the antibacterial activity of honey.