Can Sequential Human Embryo Culture Media be Used in Bovine in vitro Embryo Culture?

Çevik M., Koçyiğit A., Şen U., Kuran M.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.20, no.1, pp.149-153, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 1
  • Publication Date: 2014
  • Doi Number: 10.9775/kvfd.2013.9676
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.149-153
  • Keywords: Bovine, Embryo culture, SOF, QAM, Blastocyst quality, FERTILIZATION, MATURATION, CATTLE, SERUM, VIVO
  • Ondokuz Mayıs University Affiliated: Yes


The aim of the study was to investigate potential use of sequential human embryo culture media in culture of bovine embryos. Bovine oocytes were matured in Tissue Culture Medium-199 (TCM-199) for 22 h at 38.5 degrees C and fertilized in modified Tyrode-Albumine-Lactate-Pyruvate medium (mTALP). The putative zygotes were randomly allocated to two embryo culture media groups; (1) Synthetic Oviduct Fluid (SOF) supplemented with fatty-acid free bovine serum albumin (FAF-BSA) (8 mg/ml) and (2) sequential human embryo culture media [Quinn's Advantage Medium-(QAM)] supplemented with FAF-BSA (8 mg/ml). Zygotes were cultured in SOF and sequential QAM for 9 days (5% CO2, 5% O-2, and 90% N-2) at 38.5 degrees C. Cleavage (73.3% and 72.2%), morula (37.6% and 33.2%) and blastocysts rates (23.9% and 22.9%) were similar among groups (P>0.05), but the total blastocyst cell number were significantly higher in blastocysts developed in SOF (101.6 +/- 4.0) than those in sequential QAM (87.4 +/- 3.2) (P<0.05). QAM may be suggested to use in culture as an alternative media in terms of supporting embryo development, but low cell number in blastocysts produced in QAM may suggest a possible low pregnancy rate.