Acute Toxicity Test with Daphnia magna (Straus, 1820 Cladocera, Daphniidae) in Seydisehir Seydişehir Sediment

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Şimşek A., Şimşek K., Şişman Aydın H. G., Bakan G.

2nd International UNIDOKAP Black Sea Symposium, Samsun, Turkey, 28 - 30 November 2018, vol.1, pp.28-32

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: Samsun
  • Country: Turkey
  • Page Numbers: pp.28-32
  • Ondokuz Mayıs University Affiliated: Yes


The only chemical analysis of pollutants in ecosystems is not correct in proving the toxicological effects

of biota. Besides the chemical structure of the pollutants; ecological and toxicological effects and an

integrated study are required. Only through an integrated study can the ecotoxicological effects of

contaminating ecosystems such as sediments be accurately determined. For this reason, biological and

toxicological methods should be applied along with chemical methods for ecotoxicological studies.

Contamination in the aquatic environment disturbs the self-cleaning capacity of aquatic ecosystems,

reaching dangerous dimensions. Therefore, aquatic organisms are used for the determination of the

danger coming from the water. Organisms can be considered as a means of integrating sampling because

they live in balance with the environment surrounding them. Organisms can provide an understanding

of the nature of the relationship between contaminants in the organism and the resulting biological

effects of pollutants. In this study, a toxicity test was conducted with Daphnia magna (Straus, 1820;

Cladocera, Daphniidae), a bioindicator species commonly used in ecotoxicology.

Four different concentrations of samples were prepared from sediments of Seydişehir as 0.025 mg ml-1,

0.05 mg ml-1, 0.1 mg ml-1 and 0.2 mg ml-1, and 5 pcs of D. magna neonate were added to them. Observed

for 48 hours after the required ambient conditions were met.

D. magna were monitored at 12, 24, and 48-hour intervals, and the number of organisms living and

dying after 48 hours was determined. It was observed that the vital activities of the organisms continued

at the 12 and 24-hour controls after the test started. Acute toxicity of D. magna was determined at

Seyhişehir sediment sample after 48 hours and LC50 (lethal concentration) was found to be 0,101 mg


Keywords: Daphnia magna, ecotoxicology, sediment.