Akademik Veri Yönetim Sistemi
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, cilt.23, sa.5, ss.1051-1057, 2023 (SCI-Expanded, Scopus)
- OBJECTIVE: Gestational diabetes mellitus (GDM) is characterized by new-onset glu-cose intolerance and is most common in the sec-ond and third trimesters of pregnancy. Epigenetic modifications regulate glucose and its cellular in-teractions with metabolic pathways. Emerging ev-idence suggests that epigenetic changes contrib-ute to the pathophysiology of GDM. Since these patients have high glucose levels, the metabol-ic profiles of the fetus and the mother can affect these epigenetic changes. Therefore, we aimed to examine the potential alterations in the meth-ylation profiles of three gene promoters: the au-toimmune regulator (AIRE) gene, matrix metallo-proteinase-3 (MMP-3), and calcium voltage-gated channel subunit alpha1 G (CACNA1G).PATIENTS AND METHODS: A total of 44 pa-tients diagnosed with GDM and 20 controls were in-volved in the study. DNA isolation and bisulfite mod-ification were performed from peripheral blood sam -ples of all patients. Then, the promoter methylation status of the AIRE, MMP-3, and CACNA1G genes was determined by methylation-specific polymerase chain reaction (PCR) methylation-specific (MSP).RESULTS: Our results demonstrated that the methylation status of AIRE and MMP-3 changed to unmethylated in the GDM patients compared to healthy pregnant women (p<0.001). However, CACNA1G promoter methylation status failed to show a significant change between experimen-tal groups (p>0.05).CONCLUSIONS: Our results indicated that AIRE and MMP-3 are the genes affected by epigen-etic modification, which could be one of the caus-es of the long-term metabolic effects in maternal and fetal health and can be a target for prevention, diagnosis, or treatment for GDM in future studies.