Determination of extended spectrum ?-lactamase-producing Enterobacteriaceae in raw water buffalo milk and dairy products by conventional multiplex and real-time PCR

Gücükoğlu A., Uyanık T., Cadirci O., Uğurtay E., Kanat S., Bolukbasa A.

INTERNATIONAL DAIRY JOURNAL, vol.140, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 140
  • Publication Date: 2023
  • Doi Number: 10.1016/j.idairyj.2022.105581
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Ondokuz Mayıs University Affiliated: Yes


The prevalence of extended spectrum b-lactamase-(ESBL-) producing Enterobacteriaceae from water buffalo milk and its products, as well as genes encoding b-lactam resistance, was determined in a total of 50 raw milk samples and 120 water buffalo milk based dairy products (40 cheese, 40 cream, 30 yoghurt and 10 ice cream samples). A chromogenic-based culture method was used for isolation. Identification of strains was performed with PCR and VITEK MS. The presence of resistance genes was investigated using conventional multiplex and real-time PCR. Of the milk, cheese, yoghurt and cream samples, 26%, 17.5%, 16.6% and 12.5%, respectively, were found to contain at least one ESBL-producing Enterobacteriaceae strain; all ice cream samples were negative in terms of ESBL-producing Enterobacteriaceae. ESBL pro-duction of Enterobacter cloacae and Citrobacter freundii strains was found related to blaCTX-M gene. Four Escherichia coli isolates were found containing blaKPC gene with the coexistence of blaCTX-M and/or blaTEM.(c) 2023 Elsevier Ltd. All rights reserved.