Determination of some polycyclic aromatic hydrocarbons in filter tar of Turkish cigarettes
JOURNAL OF SEPARATION SCIENCE, vol.28, no.17, pp.2370-2373, 2005 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 28 Issue: 17
- Publication Date: 2005
- Doi Number: 10.1002/jssc.200500132
- Journal Name: JOURNAL OF SEPARATION SCIENCE
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.2370-2373
- Keywords: BaP, carcinogens, cigarette filter, DahA, filtertar, HPLC, PARTICULATE PHASE, MAINSTREAM SMOKE, PRECONCENTRATION, SEAWATER
- Ondokuz Mayıs University Affiliated: No
Abstract
A simultaneous determination method for the analysis of benzo[a]pyrene (BaP) and dibenzo[a,h]anthracene (DahA) in the filter tar of Turkish cigarettes has been developed. The method involved (a) the extraction of BaP and DahA with n-hexane from ACN solution in which the cigarette filters were extracted, and then (b) purification of the n-hexane extracts by elution on an XAD-2 column using n-hexane/dichloromethane (9: 1, v/v) mixture. Separation and quantitative determination of BaP and DahA in the extracts were carried out by HPLC and fluorescence detection on a C18 PIP column. The calculated recoveries for BaP and DahA were found in the range of 90-100% for each extraction and clean-up steps. Analysis of various filter tar of Turkish cigarettes showed that an average of 74.28 ng/filter of BaP and 5.24 ng/filter of DahA were present in Turkish cigarettes.