Simultaneous determination of nitrate, nitrite and bromate by capillary zone electrophoresis in tea infusions grown in the Black Sea region of Turkey

Tembo Z. N., Seker Aygun F., Yavuz Erdoğan B.

SEPARATION SCIENCE PLUS, vol.4, no.1, pp.45-53, 2021 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 4 Issue: 1
  • Publication Date: 2021
  • Doi Number: 10.1002/sscp.202000074
  • Journal Name: SEPARATION SCIENCE PLUS
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus
  • Page Numbers: pp.45-53
  • Keywords: bromate, capillary zone electrophoresis, nitrate, nitrite, tea infusions, HERBAL TEA, WATER, VEGETABLES, SEPARATION, BROMIDE, SAMPLES, ANIONS, IODIDE
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

A quick method for the simultaneous determination of nitrate, nitrite and bromate ions in tea infusions using capillary zone electrophoresis was developed. A three-factor full factorial design was applied for the determination of optimal conditions. Prior to the full factorial analysis, a number of experiments were conducted. A 50 mM phosphate buffer solution with pH 3.5 was selected. A capillary temperature of 40 degrees C, -20 kV applied voltage, 61.5 cm total capillary length (53.5 cm effective length) with 50 mu m i.d., and 50 mbar injection pressure for 30 s were used. The limits of detection for nitrate, nitrite, and bromate were 0.60, 0.99, and 2.14 mg/L, respectively. The anions could be detected by the method with good resolution within 7 min with average spike recovery ranging between 91.6 and 105.5% (n = 3).