Effect of IL-6-174G/C and-572G/C variants on susceptibility to osteoarthritis in Turkish population

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Yiğit S., Tekcan A., Inanir A., Nursal A. F., Akkanat S., Tural E.

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, cilt.42, ss.65-76, 2023 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 42
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/15257770.2022.2107219
  • Dergi Adı: NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Chemical Abstracts Core, Chimica, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.65-76
  • Anahtar Kelimeler: Osteoarthritis, interleukin-6 gene, variant, PCR, RFLP, INTERLEUKIN-6 GENE POLYMORPHISM, TUMOR-NECROSIS-FACTOR, KNEE OSTEOARTHRITIS, ARTICULAR CHONDROCYTES, SYNOVIAL-FLUID, DOWN-REGULATION, II COLLAGEN, IL-6 LEVELS, TNF-ALPHA, ASSOCIATION
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Osteoarthritis (OA) is a complex disorder characterized by degenerative articular cartilage in which inflammatory mechanisms play a major role in the pathogenesis. Interleukin-6 (IL6), a multifunctional cytokine, can trigger osteoclast differentiation and bone resorption. Our purpose in this study was to evaluate the association of IL-6 -174 G/C (rs1800795) and -572 G/C (rs1800796) variants with the susceptibility to OA. One hundred fifty OA patients and 150 healthy individuals were enrolled in the study. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was used for genotyping the IL-6 gene variants. The results of analyses were evaluated for statistical significance. The pain intensity was assessed using the Visual Analogue Scale (VAS). There was a statistically significant difference in the genotype and allele frequencies of the IL-6 -174 G/C variant between patients with OA and control groups (p = 0.001, p = 0.002, respectively). IL-6 -174 G/C GG genotype and G allele were more prevalent in patients with OA. We found that the IL-6 -572 G/C variant was not different between patients and controls in either genotype distribution and allele frequency. IL-6 174 G/C and -572 G/C loci GG-GG combined genotype was significantly higher in OA patients (p = 0.00). Our study suggests that there was a strong association between the IL-6 -174 G/C variant and OA in the Turkish population. Further studies on populations of different ethnic background are necessary to prove the association of IL-6 variants with OA.