Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant

---

Copy For Citation

McKain N., Genç B., Snelling T. J., Wallace R. J.

JOURNAL OF MICROBIOLOGICAL METHODS, vol.95, no.3, pp.381-383, 2013 (SCI-Expanded)

• Publication Type: Article / Article
• Volume: 95 Issue: 3
• Publication Date: 2013
• Doi Number: 10.1016/j.mimet.2013.10.009
• Journal Name: JOURNAL OF MICROBIOLOGICAL METHODS
• Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Page Numbers: pp.381-383
• Keywords: Cryoprotection, Glycerol, Rumen, Storage, PRESUMPTIVE IDENTIFICATION, DIVERSITY, RUMEN, METHANOGENS, DATABASE, CATTLE, DNA
• Ondokuz Mayıs University Affiliated: Yes

Abstract

Bacteria and archaea in frozen (-20 degrees C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected. (C) 2013 Elsevier B.V. All rights reserved.