Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant
JOURNAL OF MICROBIOLOGICAL METHODS, cilt.95, sa.3, ss.381-383, 2013 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 95 Sayı: 3
- Basım Tarihi: 2013
- Doi Numarası: 10.1016/j.mimet.2013.10.009
- Dergi Adı: JOURNAL OF MICROBIOLOGICAL METHODS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.381-383
- Anahtar Kelimeler: Cryoprotection, Glycerol, Rumen, Storage, PRESUMPTIVE IDENTIFICATION, DIVERSITY, RUMEN, METHANOGENS, DATABASE, CATTLE, DNA
- Ondokuz Mayıs Üniversitesi Adresli: Evet
Özet
Bacteria and archaea in frozen (-20 degrees C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected. (C) 2013 Elsevier B.V. All rights reserved.