JOURNAL OF MICROBIOLOGICAL METHODS, vol.95, no.3, pp.381-383, 2013 (SCI-Expanded)
Article / Article
JOURNAL OF MICROBIOLOGICAL METHODS
Science Citation Index Expanded (SCI-EXPANDED), Scopus
Cryoprotection, Glycerol, Rumen, Storage, PRESUMPTIVE IDENTIFICATION, DIVERSITY, RUMEN, METHANOGENS, DATABASE, CATTLE, DNA
Ondokuz Mayıs University Affiliated:
Bacteria and archaea in frozen (-20 degrees C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected. (C) 2013 Elsevier B.V. All rights reserved.