Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant

McKain N., Genç B., Snelling T. J., Wallace R. J.

JOURNAL OF MICROBIOLOGICAL METHODS, cilt.95, sa.3, ss.381-383, 2013 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 95 Sayı: 3
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.mimet.2013.10.009
  • Dergi Adı: JOURNAL OF MICROBIOLOGICAL METHODS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.381-383
  • Anahtar Kelimeler: Cryoprotection, Glycerol, Rumen, Storage, PRESUMPTIVE IDENTIFICATION, DIVERSITY, RUMEN, METHANOGENS, DATABASE, CATTLE, DNA
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Bacteria and archaea in frozen (-20 degrees C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected. (C) 2013 Elsevier B.V. All rights reserved.