One of the most widely utilized methods for determining seismic performance and allowing additional study is fragility analysis. The fragility curve, which is typically characterized by two-parameter log-normal distribution functions, depicts the probability of bridge components exceeding a certain damage limit. This study examines the fragility analysis of a multi-span continuous (MSC) steel roadway bridge in Türkiye. The probabilistic seismic demand model (PSDMs) is illustrated by conducting many time history analyses (THA). The nonlinear analyses are conducted for sixty earthquakes. Logarithmic regression analyses and fragility curves were derived for varying intensity measures (IM) in terms of efficiency. Monte Carlo analysis was used to derive the system fragility curve of the bridge. The PGA and ASI are the most proper intensity measure for the fragility curve of the bridge. Moreover, the slight damage can be visualized with a higher probability for the small intensity measure that even mild earthquake motion can cause some slight damage on the bridge but after slight damage bridge has further capacity until the collapse damage is visualized.