Development of an in vitro propagation protocol for Lathyrus undulatus Boiss.: A threatened species endemic to Türkiye Razvoj protokola za in vitro razmnožavanje vrste Lathyrus undulatus Boiss.: ugrožene vrste endemične za Tursku
Biologica Nyssana, cilt.17, sa.1, ss.59-67, 2026 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 17 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.46793/biolnyss.17.1.25c
- Dergi Adı: Biologica Nyssana
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.59-67
- Anahtar Kelimeler: auxins, cytokinins, ex situ conservation, micropropagation, nodal segment
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Ondokuz Mayıs Üniversitesi Adresli: Evet
Özet
Lathyrus undulatus Boiss. is a threatened plant species endemic to Türkiye from the Fabaceae family. The present study represents the first attempt to develop an in vitro propagation protocol for the conservation of this species by evaluating the effects of several cytokinins and auxins on organ development. Nodal segments from adult individuals were cultured in Murashige and Skoog (MS) medium with 6-benzylaminopurine (BAP), thidiazuron (TDZ), and N6-(2-isopentenyl)adenosine (2IP), and generated in vitro shoots were cultured on full-, half-, and quarter-strength MS medium with 3-indoleacetic acid (IAA), 1-naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA). BAP treatments produced axillary shoots with a higher number of nodes than the other treatments tested. TDZ treatments were superior to other cytokinins tested for shoot production from nodal segments, while 2IP treatments were superior for triggering shoot elongation. Rooting experiments demonstrated that L. undulatus is a problematic species to root in vitro. Shoots cultured on hormone-free MS medium and its diluted variants did not exhibit the rhizogenesis response. Likewise, shoots were found to be unresponsive to IBA treatments. NAA treatments triggered a rooting response in full-and half-strength culture medium, while IAA induced a limited rooting response in half-and quarter-strength culture medium. In the rooting phase, NAA reached a maximum root-induction rate of only 20%, which should be studied further to increase the response and ensure enhanced integration into the soil for conservation purposes. Our findings indicated that while adenine-type cytokinins are superior at inducing shoot elongation, phenylurea-type cytokinins should be favored for multiple shoot induction in L. undulatus.