Partial Purification of Glutathione S-transferase Enzyme From the Seed of Mallow (Malva Slyvestris L.) and Investigation of the Inhibition Kinetics of Some Heavy Metals

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Isik K., Taş Ö., EKİNCİ D.

PROTEIN JOURNAL, vol.42, no.4, pp.421-426, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 42 Issue: 4
  • Publication Date: 2023
  • Doi Number: 10.1007/s10930-023-10104-w
  • Journal Name: PROTEIN JOURNAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.421-426
  • Keywords: Glutathione s-transferase, Characterization, Purification, Mallow, Heavy metal, ANTIOXIDANT ENZYMES, IN-VITRO, IONS, ALUMINUM, REDUCTASE, FISH, LEAD
  • Ondokuz Mayıs University Affiliated: Yes


Glutathione S-Transferase (GST) enzyme is abundant in mammals, insects, fish and microorganisms, as well as in various tissues of these species, particularly in tissues exposed to xenobiotics from the environment. As a result, the enzyme execute detoxifying function by scavenging a diverse range of xenobiotics, such as chemotherapeutic medicines, environmental carcinogens and endogenous compounds. In this study, GST enzyme was partially purified from mallow (Malva slyvestris L.) seed for the first time and the kinetic parameters were determined. The optimum ionic intensity was found in 400 mM Tris-Buffer, optimum pH: 7.0, and optimum substrate concentration was determined as 0.2 mM. One of the biggest reasons for deterioration of ecological balance in nature is heavy metal accumulation in soil, air and water which becomes a major threat to the vital activities of living things. In this study, inhibitory effects of Cd+ 2, Ag+, Zn+ 2 and Fe+ 3 heavy metals, which are common in nature, on mallow seed glutathione S-transferase enzyme were investigated. Each heavy metal showed micromolar inhibitory effects on enzyme activity. IC50 values of the metals were calculated as 60.93, 74.602, 178.22 and 369 mu M, respectively.