Investigation of antibacterial effect of nitric oxide Ni̇tri̇k oksi̇ti̇n anti̇bakteri̇yel etki̇si̇ni̇n araştirilmasi

Çoban A. Y., Bayramoǧlu G., Ekinci B., Durupinar B.

Mikrobiyoloji Bulteni, vol.37, no.2-3, pp.151-155, 2003 (Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 2-3
  • Publication Date: 2003
  • Journal Name: Mikrobiyoloji Bulteni
  • Journal Indexes: Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.151-155
  • Keywords: Bacteria, DETA-NO, In-vitro, Nitric oxide
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

In this study, the antibacterial effects of nitric oxide (NO) have been investigated against 4 strains of multidrug-resistant Mycobacterium tuberculosis and 8 Klebsiella, 7 Escherichia coli, 7 Staphylococcus, 5 Enterobacter, 7 Pseudomonas and 7 Proteus clinical isolates by using DETA-NO ((Z)-1-[N-(2aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate) as the NO donor. Minimum inhibitory concentrations (MICs) were determined by broth microdilution method for all tested microorganisms. The MIC values found in our study were as follows; 0.25 mg/ml for all of the multidrug-resistant Mycobacterium tuberculosis strains, 1 mg/ml for Staphylococcus strains, and approximetely 2 mg/ml for the rest of the bacterial strains. These results showed that NO (or NO donor DETANO) was more effective on multidrug-resistant M.tuberculosis strains than the other bacterial species. It can be concluded that further studies are needed to explain the clinical use of NO donor DETA-NO, especially, in patients infected with multidrug-resistant M.tuberculosis.