The utility of p16INK4a and Ki-67 staining on cell blocks prepared from residual thin-layer cervicovaginal material

Akpolat I., Smith D., Ramzy I., Chirala M., Mody D.

CANCER CYTOPATHOLOGY, vol.102, no.3, pp.142-149, 2004 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 102 Issue: 3
  • Publication Date: 2004
  • Doi Number: 10.1002/cncr.20258
  • Journal Name: CANCER CYTOPATHOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.142-149
  • Keywords: p16INK4a, Ki-67, cell blocks, thinprep, cervicovaginal, high-grade squamous intraepithelial lesions, SQUAMOUS INTRAEPITHELIAL LESIONS, LIQUID-BASED SPECIMENS, UTERINE CERVIX, UNDETERMINED SIGNIFICANCE, CYTOLOGY, OVEREXPRESSION, MARKER, P16(INK4A), NEOPLASIA, BIOPSIES
  • Ondokuz Mayıs University Affiliated: No

Abstract

BACKGROUND. Cell blocks can be prepared from residual thin-layer cervicovaginal 3 (ThinPrep) material and can be used in immunohistochemical staining assays for p16INK4a and Ki-67, which are surrogate markers related to human papillomavirus 3 infection and cell proliferation, respectively. The objectives of the current study were 1) to investigate the feasibility and the role of cell block preparations in identifying significant neoplastic and preneoplastic lesions of the uterine cervix and 2) to assess the feasibility of using p16INK4a and Ki-67 immunohistochemical staining patterns on cell blocks to identify significant preneoplastic cervical lesions.