From aquatic plant to therapeutic prospect: LC-MS/MS-guided phytochemical profiling and <i>in vitro</i> antidiabetic, anticholinesterase and molecular insights into <i>Myriophyllum spicatum</i> L.
NATURAL PRODUCT RESEARCH, 2026 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Publication Date: 2026
- Doi Number: 10.1080/14786419.2026.2690074
- Journal Name: NATURAL PRODUCT RESEARCH
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, CINAHL, EMBASE, MEDLINE, Biomedical Reference Collection: Corporate Edition (EBSCO)
- Ondokuz Mayıs University Affiliated: Yes
Abstract
Myriophyllum spicatum L. methanolic extract was examined through integrated biochemical, phytochemical, computational analyses. The extract exhibited strong alpha-glucosidase inhibitory activity with an IC50 value of 198.54 & micro;g/mL, demonstrating markedly higher potency than reference inhibitor acarbose (IC50= 4738.12 & micro;g/mL), while no inhibitory activity was detected against alpha-amylase under tested conditions. In anticholinesterase assays, extract showed acetylcholinesterase inhibition with an IC50 value of 9.70 & micro;g/mL, whereas standard inhibitor donepezil exhibited a significantly stronger activity (IC50= 0.30 & micro;g/mL). No inhibitory effect was observed against butyrylcholinesterase for the extract, while donepezil displayed potent inhibition (IC50= 15.40 & micro;g/mL). Extract showed strong ABTS center dot+ scavenging activity (85.7% at 40 & micro;g/mL) but weak DPPH center dot activity (25.6% at 25 & micro;g/mL), suggesting higher affinity for hydrophilic radicals. High total phenolic, flavonoid and tannin contents correlated with antioxidant potential. LC-MS/MS revealed thirteen phenolics, mainly vanillic acid, gallic acid and cyanidin-3-O-glucoside. Docking studies supported selective alpha-glucosidase binding of vanillic acid.