Blocking E-selectin inhibits ischaemia-reperfusion-induced neutrophil recruitment to the murine testis

Celebi M., Paul A. G. A.

ANDROLOGIA, cilt.40, sa.4, ss.235-239, 2008 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 40 Sayı: 4
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1111/j.1439-0272.2008.00849.x
  • Dergi Adı: ANDROLOGIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.235-239
  • Anahtar Kelimeler: ischaemia, neutrophil, reperfusion, selectin, testis, CELL-SPECIFIC APOPTOSIS, P-SELECTIN, EXPRESSION, ENDOTOXIN, INJURY, TISSUE
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Germ cell-specific apoptosis that occurs after ischaemia-reperfusion (IR) of the murine testis is dependent on neutrophil recruitment to the testis and is dependent upon the cell adhesion molecule E-selectin. In this study, we aimed to inhibit neutrophil recruitment to the IR-induced testis. Mice were subjected to a 2-h period of testicular torsion (ischaemia) followed by detorsion (reperfusion). Shortly after onset of reperfusion, mice received either a function-blocking monoclonal anti-mouse E-selectin antibody (FBmAb) or isotype-matched control antibody. Mice were killed 24 h after reperfusion and cells isolated from the testis were analysed for the presence of neutrophil infiltration by flow cytometry. Administration of FBmAb inhibited neutrophil recruitment to the IR-induced testis dramatically. Therefore, blockage of E-selectin may be a strategy to treat post-ischaemic testis.