Blocking E-selectin inhibits ischaemia-reperfusion-induced neutrophil recruitment to the murine testis

Celebi M., Paul A. G. A.

ANDROLOGIA, vol.40, no.4, pp.235-239, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 40 Issue: 4
  • Publication Date: 2008
  • Doi Number: 10.1111/j.1439-0272.2008.00849.x
  • Journal Name: ANDROLOGIA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.235-239
  • Keywords: ischaemia, neutrophil, reperfusion, selectin, testis, CELL-SPECIFIC APOPTOSIS, P-SELECTIN, EXPRESSION, ENDOTOXIN, INJURY, TISSUE
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

Germ cell-specific apoptosis that occurs after ischaemia-reperfusion (IR) of the murine testis is dependent on neutrophil recruitment to the testis and is dependent upon the cell adhesion molecule E-selectin. In this study, we aimed to inhibit neutrophil recruitment to the IR-induced testis. Mice were subjected to a 2-h period of testicular torsion (ischaemia) followed by detorsion (reperfusion). Shortly after onset of reperfusion, mice received either a function-blocking monoclonal anti-mouse E-selectin antibody (FBmAb) or isotype-matched control antibody. Mice were killed 24 h after reperfusion and cells isolated from the testis were analysed for the presence of neutrophil infiltration by flow cytometry. Administration of FBmAb inhibited neutrophil recruitment to the IR-induced testis dramatically. Therefore, blockage of E-selectin may be a strategy to treat post-ischaemic testis.