Five actinobacteria isolates, KC201(T), KC401, KC310(T), KC712(T) and 6K102(T), were recovered from the Karakum Desert during an investigation of novel actinobacteria with biotechnological potential. A polyphasic approach confirmed the affiliation of the strains to the genus Nonomuraea. The strains showed chemotaxonomic and morphological properties consistent with their classification in the genus Nonomuraea. Furthermore, these strains clearly distinguished and formed well supperted clades in phylogenetic and phylogenomic trees. Low ANI and dDDH values and distinguishing phenotypic properties between isolates KC201(T), KC310(T), KC712(T) and 6K102(T) showed that these strains belonged to novel Nonomuraea species, the names proposed for these taxa are Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov., with the type strains KC310(T) (=CGMCC 4.7331(T) =DSM 102919(T) =KCTC 39774(T)), KC712(T) (=CGMCC 4.7334(T) =DSM 102925(T) =KCTC 39776(T)), KC201(T) (=CGMCC 4.7339(T) =DSM 102917(T) =KCTC 39781(T)) and 6K102(T) (=CGMCC 4.7541(T) =JCM 32916(T)), respectively.