Voltammetric Study on Interaction of Cysteine with Type I-Collagen in the Aqueous Medium
RUSSIAN JOURNAL OF ELECTROCHEMISTRY, cilt.47, sa.7, ss.805-810, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 47 Sayı: 7
- Basım Tarihi: 2011
- Doi Numarası: 10.1134/s1023193511070123
- Dergi Adı: RUSSIAN JOURNAL OF ELECTROCHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.805-810
- Anahtar Kelimeler: type I-collagen, cysteine, interaction, voltammetry, STRIPPING VOLTAMMETRY, MERCURY-ELECTRODES, GOLD ELECTRODE, ADSORPTION, POLAROGRAPHY, OXIDATION, IONS
- Ondokuz Mayıs Üniversitesi Adresli: Evet
Özet
Interaction of cysteine with type I-collagen from bovine achilles tendon in the aqueous solutions has been examined using square wave voltammetry (SWV) and cyclic voltammetry (CV) techniques. In the absence of cysteine, type I-collagen gives a reversible peak at -0.114 V in Britton-Robinson (B-R) buffer (pH 4.0). The electrochemical parameters (I(p)/f, E(p)/f, E(p)/pH, I(p)/pH, I(p)/v, I(p)/v(1/2)) of type I-collagen have been also studied. In addition, it has been determined that there is a linear relationship between current and concentration of type I-collagen. On the other hand, cysteine exhibits a reversible peak at -0.383 V due to the reduction of mercurous cysteine thiolate. By using a hanging mercury drop electrode in aqueous solutions, SWV and CV voltammograms obtained for type I-collagen in the presence of cysteine indicate that there is an interaction between type I-collagen and cysteine. In the presence of cysteine, peak current of type I-collagen decreases and a new peak is observed at -0.121 V for cysteine which is bonded to type I-collagen.