Development of new total RNA isolation method for tissues with rich phenolic compounds

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Secgin Z., Gokdemir G., Atabay E. S., Kurt Kızıldoğan A., Kavas M.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.45, no.4, pp.343-350, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 4
  • Publication Date: 2020
  • Doi Number: 10.1515/tjb-2019-0375
  • Journal Name: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.343-350
  • Keywords: CTAB, hazelnut, phenolics, RNA isolation, RNA sequencing, HIGH-QUALITY RNA, PLANT-TISSUES, WOODY-PLANTS, EXTRACTION, POLYSACCHARIDES
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

Background: RNAs to be used in transcriptome analysis must be of high quality and pure in order to ensure maximum representation of the expressed genes. RNA isolation is difficult in hazelnut tissues containing large amounts of secondary metabolite, phenolic compounds and the cell wall structure. Commonly used protocols for RNA isolation are those that require a lot of labor and time and also do not allow sufficient RNA isolation when applied to tissues rich in phenolic compounds. This study was aimed to develop an efficient method for isolation of total RNAs from bud of hazelnut to be used in RNA sequencing.