Research Information System
ANKARA INTERNATIONAL CONGRESS ON SCIENTIFIC RESEARCH-IX, 26 - 29 December 2023, pp.1924-1925, (Summary Text)
Bacter
a are m
croorgan
sms that can surv
ve
n var
ous hab
tats,
nclud
ng extreme env
ronments. These bacter
a
nclude psychroph
l
c and psychrotolerant bacter
a, wh
ch have adapted to low temperatures and can be used for var
ous purposes such as enzyme product
on, b
olog
cal control and removal of env
ronmental wastes. Unt
l now, no study has been conducted on the bacter
al b
od
vers
ty of the Kaçkar Mounta
ns, located
n the northeast of Turkey, where var
ous land and water ecosystems,
nclud
ng glac
al lakes, are located. In th
s study, water, sed
ment and so
l samples were collected from n
ne d
fferent po
nts
n the Kaçkar Mounta
ns w
th
n the borders of R
ze Prov
nce, and psychroph
l
c/psychrotolerant bacter
a were
solated from these samples. 7 d
fferent med
a were used for
solat
on, and the petr
d
shes
noculated w
th the spread
ng plate method were
ncubated at 10°C for four weeks. The colon
es obta
ned at the end of the
ncubat
on were pur
f
ed by the streak plate method and 377
solates were obta
ned as pure cultures. The 16S rRNA gene of 46 representat
ve
solates, selected accord
ng to the
r morpholog
cal character
st
cs among the pur
f
ed
solates, was sequenced and analyzed. As a result of the analys
s, 46
solates were d
str
buted
nto 15 d
fferent genera. 18
solates were ass
gned as Pseudomonas, 7
solates were Bacllus, 4
solates were Janthnobacterum, 3
solates were Flavobacterum, 2
solates each were Pedobacter, Serrata
and Exguobacterum, and 1
solate each was a member of the genera Aeromonas, Lefsona,
Okbacterum, Streptomyces, Carnobacterum, Rhodococcus, Denococcus and Pantoea. It was determ
ned that an
solate represented a new spec
es w
th
n the Pedobacter genus as
t showed 98.61% s
m
lar
ty value
n terms of 16S rRNA gene sequence w
th the most closely related type stra
n. The
solates obta
ned
n the study were screened on sol
d med
a for extracellular amylase, l
pase, protease and ch
t
nase product
on, and 22
solates gave pos
t
ve results for l
pase and protease, 11
solates for amylase and 8
solates for ch
t
nase enzyme. The
solates were also screened for s
derophore product
on
n un
versal CAS med
um and 13
solates were found to g
ve pos
t
ve results. Th
s study revealed the bacter
al b
od
vers
ty
n the Kaçkar Mounta
ns and the b
otechnolog
cal potent
al of th
s d
vers
ty for the f
rst t
me. As a result,
t has been shown that the Kaçkar Mounta
ns are r
ch
n psychroph
l
c/psychrotolerant bacter
al b
od
vers
ty and that th
s d
vers
ty can benef
t from a b
otechnolog
cal perspect
ve.