Occurrence of soil-borne sugar beet viruses transmitted by Polymyxa betae northern and central Turkey

Kutluk Yılmaz N. D., Sökmen M.

Journal of Plant Pathology, vol.92, no.2, pp.507-510, 2010 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 92 Issue: 2
  • Publication Date: 2010
  • Journal Name: Journal of Plant Pathology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.507-510
  • Keywords: BNYVV, BSBV, BVQ, ELISA, P. betae, RT-PCR, Survey
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

A survey was conducted to determine the incidence of Beet necrotic yellow vein virus (BNYVV), Beet soilborne virus (BSBV) and Beet virus Q (BVQ) and their vector Polymyxa betae in sugar beet fields in central and northern Turkey. A total of 510 soil samples were collected in 2004 and 2005. Three detection techniques were used: ELISA for BNYVV and BSBV, RT-PCR for BVQ and direct staining of bait plant root tissues for P. betae cystosori. Bait plant tests and ELISA showed that 54.3% of the soils contained viruliferous P. betae cystosori. BSBV single infections were the most prevalent (23.7%) followed by double infection with BNYVV (23.1%), and single infection of BNYVV (7.5%). It was also found that 31.4% of the roots were infested with aviruliferous P. betae cystosori while 14.3% of the roots did not contain any cystosori. Forty five samples were selected on the basis of their geographical locations and tested for the presence of BVQ by RT-PCR. However, BVQ was not detected in any sugar beet samples tested.