Gene expression analysis of bud burst process in European hazelnut (Corylus avellana L.) using RNA-Seq

Creative Commons License

Kavas M., Kurt Kızıldoğan A., Balik H. I.

PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, vol.25, no.1, pp.13-29, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 1
  • Publication Date: 2019
  • Doi Number: 10.1007/s12298-018-0588-2
  • Journal Name: PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.13-29
  • Keywords: Bud burst, Dormancy, Hazelnut, Phenylpropanoid metabolism, Phytohormone biosynthesis, RNA-Seq, PHENYLPROPANOID METABOLISM, TRANSCRIPTOME ANALYSIS, DORMANCY INDUCTION, FLOWER BUDS, GENOME-WIDE, CROWN BUDS, BIOSYNTHESIS, DIVERSITY, FAMILY, REQUIREMENTS
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

The control of bud burst process depending on temperature is crucial factor in woody perennial plants to survive in unfavorable ecological conditions. Although it has important economic and agronomic values, little information is available on the molecular mechanism of the bud burst process in Corylus avellana. Here for the first time, we conducted a de novo transcriptome-based experiment using eco-dormant leaf bud tissues. Four transcriptome libraries were constructed from the leaf bud tissues and sequenced via Illumina platform. Transcriptome analysis revealed 86,394 unigenes with a mean length of 1189 nt and an N50 of 1916 nt. Among these unigenes, 63,854 (73.78%) of them were annotated by at least one database. De novo assembled transcripts were enriched in phenylpropanoid metabolism, phytohormone biosynthesis and signal transduction pathways. Analyses of phytohormone-associated genes revealed important changesduring bud burst, in response to gibberellic acid, auxin, and brassinosteroids. Approximately 2163 putative transcription factors were predicted, of which the largest number of unique transcripts belonged to the MYB transcription factor family. These results contribute to a better understanding of the regulation of bud burst genes in perennial plants.