Research Information System
Evidence-based Complementary and Alternative Medicine, vol.2018, 2018 (SCI-Expanded)
Galactites is a genus of flowering plants belonging to Asteraceae family. This genus is mainly represented by the Galactites elegans (All.) Nyman ex Soldano, the milky thistle, a plant of Mediterranean origin. Galactites elegans is consumed as a monofloral boar thistle honey. Chromatography separation of CHCl 3 and n-BuOH extracts of aerial parts of G. elegans led to isolation of 18 pure compounds. Their structures were elucidated by 1D-and 2D-NMR spectroscopy and confirmed by mass spectrometry analysis. Sinapic aldehyde, abietin, chlorogenic acid, neochlorogenic acid, 8-hydroxypinoresinol, 9-hydroxypinoresinol, pinoresinol, 4-ketopinoresinol, nortrachelogenin, and erythro-guaiacylglycerol-O-4-dihydroconiferyl alcohol were isolated from CHCl 3 extract, while luteolin 4-O-glucuronide, naringenin-7-O-neohesperidoside, kaempferol-3-O-L-rhamnopyranosyl-(1→6)-D-glucopyranoside, apigenin-7-O-L-rhamnopyranosyl-(1→6)-D-glucopyranoside, quercitrin, quercetin-3-O-L-rhamnopyranosyl-(1→6)-D-glucopyranoside, ciwujiatone, and nortrachelogenin-4,4-di-O-D-glucopyranoside were obtained from n-BuOH extract. The majority of isolated compounds displayed a significant antioxidant potential in vitro test (DPPH). The ability of compounds to reduce the level of peroxides in control and BHP-treated Jurkat cells was studied. The lignan derivatives were also able to reduce at 50 M the basal level of peroxides in Jurkat cells as well as counteract peroxide increase induced by BHP treatment. Particularly 8-hydroxypinoresinol was the most active showing 70% of peroxide level inhibition.