Genomic insight into Myroides oncorhynchi sp. nov., a new member of the Myroides genus, isolated from the internal organ of rainbow trout (Oncorhynchus mykiss)

SATICIOĞLU İ. B., AY H., ALTUN S., KARAKAYA E., Gunduz E. S., Aydogdu D., ...More

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, vol.116, no.3, pp.291-302, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 116 Issue: 3
  • Publication Date: 2023
  • Doi Number: 10.1007/s10482-022-01804-x
  • Journal Name: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.291-302
  • Keywords: Aquaculture, Rainbow trout, Myroides, Novel species, Overall genome-related index, FLAVOBACTERIUM-ODORATUM STUTZER, PROPOSED MINIMAL STANDARDS, EMENDED DESCRIPTION, IDENTIFICATION, SEQUENCES, DATABASE, FAMILY, GENUS
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

The strain M-43(T) was isolated from the Oncorhynchus mykiss from a fish farm in Mugla, Turkey. Pairwise 16S rRNA gene sequence analysis was used to identify strain M-43(T). The strain was a member of the genus Myroides sharing the highest 16S rRNA gene sequence identity levels of 98.7%, 98.3%, and 98.3% with the type strains of M. profundi D25(T), M. odoratimimus subsp. odoratimimus CCUG 39352(T) and M. odoratimimus subsp. xuanwuensis DSM27251(T), respectively. A polyphasic taxonomic approach including whole genome-based analyses was employed to confirm the taxonomic provenance of strain M-43(T) within the genus Myroides. The overall genome relatedness indices (OGRI) for strain M-43(T) compared with its most closely related type strains M. odoratimimus subsp. xuanwuensis DSM 27251(T), M. profundi D25(T), and M. odoratimimus subsp. odoratimimus ATCC BAA-634(T), were calculated as 25.3%, 25.1%, and 25% for digital DNA-DNA hybridization (dDDH), 83.3%, 83.6%, and 83.4% for average nucleotide identity (ANI) analyses, respectively. The OGRI values between strain M-43(T) and its close neighbors confirmed that the strain represents a novel species in the genus Myroides. The DNA G + C content of the strain is 33.7%. The major fatty acids are iso-C-15:0 and summed feature 9 (iso-C-17:1 omega 9c and/or 10-methyl C-16:0). The predominant polar lipids are phosphatidylethanolamine, an amino-lipid and five unidentified lipids. The major respiratory quinone is MK-6. Chemotaxonomic and phylogenomic analyses of this isolate confirmed that the strain represents a novel species for which the name Myroides oncorhynchi sp. nov. is proposed, with M-43(T) as the type strain (JCM 34205(T) = KCTC 82265(T)).