A Cytokinin Analog Thidiazuron Suppresses Shoot Growth in Potted Rose Plants via the Gibberellic Acid Pathway

Creative Commons License

Celikel F. G., Zhang Q., Zhang Y., Reid M. S., Jiang C.

FRONTIERS IN PLANT SCIENCE, cilt.12, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12
  • Basım Tarihi: 2021
  • Doi Numarası: 10.3389/fpls.2021.639717
  • Dergi Adı: FRONTIERS IN PLANT SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Directory of Open Access Journals
  • Anahtar Kelimeler: ethylene, GA oxidase enzymes, gene expression, internode length and thickness, microscopy, miniature rose, plant height, thidiazuron, ETHYLENE BIOSYNTHESIS, LEAF SENESCENCE, CROSS-TALK, ARABIDOPSIS, STEM, INHIBITORS, SEEDLINGS
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Application of thidiazuron (N-phenyl-N '-1,2,3-thiadiazol-5-ylurea, TDZ), a cytokinin analog, to inhibit the leaf yellowing that occurs after pinching potted rose plants, resulted in compact plants with shorter shoots and thicker internodes. Two weeks after treatment with 100 mu M of TDZ, new shoots were half as long as those in control plants, and stem diameters were about 40% greater. This effect of TDZ is associated with changes in cell architecture. Although TDZ treatment stimulated ethylene production by the plants, inhibitors of ethylene biosynthesis (2-aminoethoxyvinyl glycine) or action (silver thiosulfate) did not affect the response of plants to TDZ. We found that TDZ treatment significantly suppressed the expression of bioactive gibberellic acid (GA) biosynthesis genes encoding GA3 and GA20 oxidases and slightly increased the expression of GA catabolism genes encoding GA2 oxidase. Application of GA(3) and TDZ together resulted in normal elongation growth, although stem diameters were still somewhat thicker. Our results suggest that TDZ regulates shoot elongation and stem enlargement in potted rose plants through the modulation of bioactive GA biosynthesis.