The genus Echinops belonging to the Asteraceae family comprises 130 species. The dried leaves and seeds of Echinops orientalis Trauv. were extracted separately with methanol. Apigenin-7-0-(6″-trans-p-coumaroyl-β-D-glucopyranoside 1, and Apigenin-7-0-β-D glucoside 2 were isolated from leaves and 1-methoxycarbonylindole 3 and beta-sitositerol 4 were isolated from seeds. The compounds were isolated by chromatographic techniques, based on column chromatography, preparative TLC and identified by spectroscopic methods including ID-, 2D-NMR, UV, IR, HPLC-QTOF/MS. Isolated compounds and extracts were applied to antioxidant activity tests. While seeds and leaves extracts have high DPPH and moderate ABTS radical scavenging activities, the isolated flavones exhibited high cation radical scavenging activities. © 2014 ACG Publications. All rights reserved.